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lnrM [2019-06-26 07:45:59]
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lnrM [2019-06-26 07:45:59]

similar to [SW|ABC transporter] (membrane protein), involved in resistance to linearmycin
locus
BSU08320
pI
9.62
mw
42.95 kDa
protein length
396 aa Sequence Blast
gene length
1191 bp Sequence Blast
function
resistance to linearmycin
product
[SW|ABC transporter] (membrane protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    906,764 907,954

    Phenotypes of a mutant

  • overexpression of [gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM] and [gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN] stimulates [SW|biofilm formation ][pubmed|28461449]

    • The protein

    Protein family

  • ABC-2 integral membrane protein family (according to UniProt)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]-[gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]-[gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN]
    description
    [Pubmed|26647299]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]: activation, [Pubmed|26647299], in [regulon|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK regulon]

    • regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
  • induced in the presence of linearmycin and other polyenes ([protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]) [pubmed|32973732,28461449]
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    Biological materials

    Mutant

  • MGNA-C302 (yfiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2300 NBRP B. subtilis, Japan]
  • BKE08320 ([gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC
  • BKK08320 ([gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC

    • References

  • 10092453,26647299,28461449